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lc3 1 1000 14600 1 ap proteintech  (Proteintech)


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    Proteintech lc3 1 1000 14600 1 ap proteintech
    Lc3 1 1000 14600 1 Ap Proteintech, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 2126 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lc3+proteintech/LC3+Antibody/10__1038_slash_s42003___026___11069___z-279-15-17
    Average 96 stars, based on 2126 article reviews
    lc3 1 1000 14600 1 ap proteintech - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: S100A11 regulates microglial inflammatory response in neuropathic pain via H3K27ac-TFEB-mitochondrial autophagy axis
    Article Snippet: Protein samples (30–40 μg) were separated by SDS-PAGE at 90 V for 90 min and transferred onto PVDF membranes (Millipore, USA) via wet transfer (200 mA, 90 min). .. Membranes were blocked with 5% skim milk for 60 min, rinsed with TBST, and incubated with primary antibodies at 4 °C overnight (β-actin: Proteintech, 66009-1-Ig, 1:10000; S100A11: Abcam, ab169530, 1:1000; NLRP3: AdipoGen, AG-20B-0014, 1:1000; GSDMD-N: Abcam, ab215203, 1:1000; ASC: Wanleibio, WL02462, 1:500; Caspase1: CST, 89332, 1:1000; Bax: Proteintech, 50599-2-Ig, 1:2000; Bcl-2: abclone, A19693, 1:2000; COXIV: Proteintech, 11242-1-AP, 1:1000; LC3: Proteintech, 14600-1-AP, 1:2000; P62: Proteintech, 18420-1-AP, 1:2000; Parkin: Proteintech, 14060-1-AP, 1:1000; Pink1: Proteintech, 23274-1-AP, 1:2000; H3: Proteintech, 17168-1-AP, 1:2000; TFEB: Proteintech, 13372-1-AP, 1:1000; TFEC: Proteintech, 13547-1-AP, 1:1000; TFE3: Proteintech, 14480-1-AP, 1:1000; H3K9ac: CST, 9649, 1:1000; H3K18ac: CST, 13998, 1:1000; H3K27ac: CST, 8173, 1:1000; H4K12ac; CST, 13944, 1:1000; H4K16ac: CST, 13534, 1:1000). ..

    Article Title: S100A11 regulates microglial inflammatory response in neuropathic pain via H3K27ac-TFEB-mitochondrial autophagy axis.
    Article Snippet: Protein samples (30–40 μg) were separated by SDS-PAGE at 90 V for 90 min and transferred onto PVDF membranes (Millipore, USA) via wet transfer (200 mA, 90 min). .. Membranes were blocked with 5% skim milk for 60 min, rinsed with TBST, and incubated with primary antibodies at 4 °C overnight (β-actin: Proteintech, 66009-1-Ig, 1:10000; S100A11: Abcam, ab169530, 1:1000; NLRP3: AdipoGen, AG-20B-0014, 1:1000; GSDMD-N: Abcam, ab215203, 1:1000; ASC: Wanleibio, WL02462, 1:500; Caspase1: CST, 89332, 1:1000; Bax: Proteintech, 50599-2-Ig, 1:2000; Bcl-2: abclone, A19693, 1:2000; COXIV: Proteintech, 11242-1-AP, 1:1000; LC3: Proteintech, 14600-1-AP, 1:2000; P62: Proteintech, 18420-1-AP, 1:2000; Parkin: Proteintech, 14060-1-AP, 1:1000; Pink1: Proteintech, 23274-1-AP, 1:2000; H3: Proteintech, 17168-1-AP, 1:2000; .. TFEB: Proteintech, 13372-1-AP, 1:1000; TFEC: Proteintech, 13547-1-AP, 1:1000; TFE3: Proteintech, 14480-1- AP, 1:1000; H3K9ac: CST, 9649, 1:1000; H3K18ac: CST, 13998, 1:1000; H3K27ac: CST, 8173, 1:1000; H4K12ac; CST, 13944, 1:1000; H4K16ac: CST, 13534, 1:1000).

    other:

    Article Title: Resveratrol delays senescence of human dental pulp stem cells via activating the SIRT1-mitochondrial autophagy.
    Article Snippet: Horseradish peroxidase-labeled secondary antibody (1:5000, Abcam, Cambridge, UK) was employed to treat proteins for 2 h at room temperature.

    Western Blot:

    Article Title: Nobiletin mitigates NAFLD via lipophagy and inflammation.
    Article Snippet: Nonalcoholic fatty liver disease (NAFLD), an increasingly serious health issue around the world, is characterized as a lipid metabolic disorder without any satisfactory treatment.. Nobiletin (NOB), a citrus flavonoid, is considered a promising candidate for NAFLD prevention although there is limited research towards its exact mechanism.. In this study, the preventative effect of NOB on NAFLD was investigated using high fat diet-fed ApoE mice and free fatty acid-treated HepG2 cells.



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    Knockdown of S100A11 improves inflammatory response and pyroptosis in primary microglia by inhibiting mitophagy. A Western blot analysis of Pink1, Parkin, P62, LC3II protein levels of primary microglia; B-E Relative levels normalized to COXIV were quantified (for three independent experiments, statistical significance was determined by two-way ANOVA with appropriate post hoc tests). G-H Labeling of mitochondria in primary microglia with Tom20 (red); immunofluorescence staining for <t>LC3B</t> (green). Quantification of the mean fluorescence intensity of <t>LC3</t> is shown in panels F and I . (Data are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA with appropriate post hoc tests) for three independent experiments. Immunofluorescence staining of CD68 ( J ) and CD206 ( K ) in microglia (red); green fluorescence indicates phalloidin. Quantification of relative fluorescence intensities is shown in panels. L , M ( Data are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA with appropriate post hoc tests), for three independent experiments. Q Western blot analysis of NLRP3 protein levels of microglia; N Relative levels normalized to β-actin was quantified (for three independent experiments, statistical significance was determined by one-way ANOVA with appropriate post hoc tests). Immunofluorescence staining of NLRP3 ( R ) and Caspase1 ( S ) in microglia (red); green fluorescence indicates phalloidin. Quantification of relative fluorescence intensities is shown in panels O , P (Statistical significance was determined by one-way ANOVA with appropriate post hoc tests) for three independent experiments. Scale bars as indicated. Data are presented as mean ± SEM. * P < 0.05 , ** P < 0.01 , *** P < 0.001
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    Image Search Results


    Knockdown of S100A11 improves inflammatory response and pyroptosis in primary microglia by inhibiting mitophagy. A Western blot analysis of Pink1, Parkin, P62, LC3II protein levels of primary microglia; B-E Relative levels normalized to COXIV were quantified (for three independent experiments, statistical significance was determined by two-way ANOVA with appropriate post hoc tests). G-H Labeling of mitochondria in primary microglia with Tom20 (red); immunofluorescence staining for LC3B (green). Quantification of the mean fluorescence intensity of LC3 is shown in panels F and I . (Data are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA with appropriate post hoc tests) for three independent experiments. Immunofluorescence staining of CD68 ( J ) and CD206 ( K ) in microglia (red); green fluorescence indicates phalloidin. Quantification of relative fluorescence intensities is shown in panels. L , M ( Data are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA with appropriate post hoc tests), for three independent experiments. Q Western blot analysis of NLRP3 protein levels of microglia; N Relative levels normalized to β-actin was quantified (for three independent experiments, statistical significance was determined by one-way ANOVA with appropriate post hoc tests). Immunofluorescence staining of NLRP3 ( R ) and Caspase1 ( S ) in microglia (red); green fluorescence indicates phalloidin. Quantification of relative fluorescence intensities is shown in panels O , P (Statistical significance was determined by one-way ANOVA with appropriate post hoc tests) for three independent experiments. Scale bars as indicated. Data are presented as mean ± SEM. * P < 0.05 , ** P < 0.01 , *** P < 0.001

    Journal: The Journal of Headache and Pain

    Article Title: S100A11 regulates microglial inflammatory response in neuropathic pain via H3K27ac-TFEB-mitochondrial autophagy axis

    doi: 10.1186/s10194-026-02328-9

    Figure Lengend Snippet: Knockdown of S100A11 improves inflammatory response and pyroptosis in primary microglia by inhibiting mitophagy. A Western blot analysis of Pink1, Parkin, P62, LC3II protein levels of primary microglia; B-E Relative levels normalized to COXIV were quantified (for three independent experiments, statistical significance was determined by two-way ANOVA with appropriate post hoc tests). G-H Labeling of mitochondria in primary microglia with Tom20 (red); immunofluorescence staining for LC3B (green). Quantification of the mean fluorescence intensity of LC3 is shown in panels F and I . (Data are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA with appropriate post hoc tests) for three independent experiments. Immunofluorescence staining of CD68 ( J ) and CD206 ( K ) in microglia (red); green fluorescence indicates phalloidin. Quantification of relative fluorescence intensities is shown in panels. L , M ( Data are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA with appropriate post hoc tests), for three independent experiments. Q Western blot analysis of NLRP3 protein levels of microglia; N Relative levels normalized to β-actin was quantified (for three independent experiments, statistical significance was determined by one-way ANOVA with appropriate post hoc tests). Immunofluorescence staining of NLRP3 ( R ) and Caspase1 ( S ) in microglia (red); green fluorescence indicates phalloidin. Quantification of relative fluorescence intensities is shown in panels O , P (Statistical significance was determined by one-way ANOVA with appropriate post hoc tests) for three independent experiments. Scale bars as indicated. Data are presented as mean ± SEM. * P < 0.05 , ** P < 0.01 , *** P < 0.001

    Article Snippet: Membranes were blocked with 5% skim milk for 60 min, rinsed with TBST, and incubated with primary antibodies at 4 °C overnight (β-actin: Proteintech, 66009-1-Ig, 1:10000; S100A11: Abcam, ab169530, 1:1000; NLRP3: AdipoGen, AG-20B-0014, 1:1000; GSDMD-N: Abcam, ab215203, 1:1000; ASC: Wanleibio, WL02462, 1:500; Caspase1: CST, 89332, 1:1000; Bax: Proteintech, 50599-2-Ig, 1:2000; Bcl-2: abclone, A19693, 1:2000; COXIV: Proteintech, 11242-1-AP, 1:1000; LC3: Proteintech, 14600-1-AP, 1:2000; P62: Proteintech, 18420-1-AP, 1:2000; Parkin: Proteintech, 14060-1-AP, 1:1000; Pink1: Proteintech, 23274-1-AP, 1:2000; H3: Proteintech, 17168-1-AP, 1:2000; TFEB: Proteintech, 13372-1-AP, 1:1000; TFEC: Proteintech, 13547-1-AP, 1:1000; TFE3: Proteintech, 14480-1-AP, 1:1000; H3K9ac: CST, 9649, 1:1000; H3K18ac: CST, 13998, 1:1000; H3K27ac: CST, 8173, 1:1000; H4K12ac; CST, 13944, 1:1000; H4K16ac: CST, 13534, 1:1000).

    Techniques: Knockdown, Western Blot, Labeling, Immunofluorescence, Staining, Fluorescence

    S100A11 knockdown alleviates microglial inflammation by inhibiting mitophagy through the TFEB/H3K27ac axis. A-B Western blot analysis of TFEB, TFEC, and TFE3 protein expression levels in the cytoplasm and nucleus of primary microglia, along with the corresponding detection and quantification plots ( D-F , for three independent experiments, statistical significance was determined by one-way ANOVA with appropriate post hoc tests). C Immunofluorescence staining of TFEB in primary microglia. G Western blot analysis of pink1, Parkin, P62, LC3II protein levels of primary microglia; H-K Relative levels normalized to COX IV were quantified (for three independent experiments, statistical significance was determined by one-way ANOVA with appropriate post hoc tests). M Labeling of mitochondria (red) in primary microglia with Tom20; immunofluorescence staining for LC3B (green). Quantification of the mean fluorescence intensity of LC3 is shown in panel L . and relative fluorescence intensity was quantified in panel L ( n = 3). N Western blot analysis of H4K16ac, H4K12ac, H3K27ac, H3K18ac, H3K9ac protein levels of microglia; O-S Relative levels normalized to histone H3 were quantified (for three independent experiments, statistical significance was determined by two-way ANOVA with appropriate post hoc tests). T Western blot analysis of Pink1, Parkin, P62, LC3II protein levels of primary microglia; U-X Relative levels normalized to COX IV were quantified (for three independent experiments, statistical significance was determined by one-way ANOVA with appropriate post hoc tests). Z Labeling of mitochondria (red) in primary microglia with Tom20; immunofluorescence staining for LC3B (green), and relative fluorescence intensity was quantified in panel Y ( n = 3). Scale bars as indicated. Data are presented as mean ± SEM. * P < 0.05 , ** P < 0.01 , *** P < 0.001

    Journal: The Journal of Headache and Pain

    Article Title: S100A11 regulates microglial inflammatory response in neuropathic pain via H3K27ac-TFEB-mitochondrial autophagy axis

    doi: 10.1186/s10194-026-02328-9

    Figure Lengend Snippet: S100A11 knockdown alleviates microglial inflammation by inhibiting mitophagy through the TFEB/H3K27ac axis. A-B Western blot analysis of TFEB, TFEC, and TFE3 protein expression levels in the cytoplasm and nucleus of primary microglia, along with the corresponding detection and quantification plots ( D-F , for three independent experiments, statistical significance was determined by one-way ANOVA with appropriate post hoc tests). C Immunofluorescence staining of TFEB in primary microglia. G Western blot analysis of pink1, Parkin, P62, LC3II protein levels of primary microglia; H-K Relative levels normalized to COX IV were quantified (for three independent experiments, statistical significance was determined by one-way ANOVA with appropriate post hoc tests). M Labeling of mitochondria (red) in primary microglia with Tom20; immunofluorescence staining for LC3B (green). Quantification of the mean fluorescence intensity of LC3 is shown in panel L . and relative fluorescence intensity was quantified in panel L ( n = 3). N Western blot analysis of H4K16ac, H4K12ac, H3K27ac, H3K18ac, H3K9ac protein levels of microglia; O-S Relative levels normalized to histone H3 were quantified (for three independent experiments, statistical significance was determined by two-way ANOVA with appropriate post hoc tests). T Western blot analysis of Pink1, Parkin, P62, LC3II protein levels of primary microglia; U-X Relative levels normalized to COX IV were quantified (for three independent experiments, statistical significance was determined by one-way ANOVA with appropriate post hoc tests). Z Labeling of mitochondria (red) in primary microglia with Tom20; immunofluorescence staining for LC3B (green), and relative fluorescence intensity was quantified in panel Y ( n = 3). Scale bars as indicated. Data are presented as mean ± SEM. * P < 0.05 , ** P < 0.01 , *** P < 0.001

    Article Snippet: Membranes were blocked with 5% skim milk for 60 min, rinsed with TBST, and incubated with primary antibodies at 4 °C overnight (β-actin: Proteintech, 66009-1-Ig, 1:10000; S100A11: Abcam, ab169530, 1:1000; NLRP3: AdipoGen, AG-20B-0014, 1:1000; GSDMD-N: Abcam, ab215203, 1:1000; ASC: Wanleibio, WL02462, 1:500; Caspase1: CST, 89332, 1:1000; Bax: Proteintech, 50599-2-Ig, 1:2000; Bcl-2: abclone, A19693, 1:2000; COXIV: Proteintech, 11242-1-AP, 1:1000; LC3: Proteintech, 14600-1-AP, 1:2000; P62: Proteintech, 18420-1-AP, 1:2000; Parkin: Proteintech, 14060-1-AP, 1:1000; Pink1: Proteintech, 23274-1-AP, 1:2000; H3: Proteintech, 17168-1-AP, 1:2000; TFEB: Proteintech, 13372-1-AP, 1:1000; TFEC: Proteintech, 13547-1-AP, 1:1000; TFE3: Proteintech, 14480-1-AP, 1:1000; H3K9ac: CST, 9649, 1:1000; H3K18ac: CST, 13998, 1:1000; H3K27ac: CST, 8173, 1:1000; H4K12ac; CST, 13944, 1:1000; H4K16ac: CST, 13534, 1:1000).

    Techniques: Knockdown, Western Blot, Expressing, Immunofluorescence, Staining, Labeling, Fluorescence